γ-Aminobutyric acid (4-Aminobutyric acid) 是成年哺乳动物大脑中主要的抑制性神经递质，能够与离子移变GABAA受体和促代谢GABAB受体结合发挥作用。γ-Aminobutyric acid 能够阻断中枢神经系统的特定信号，并带来镇静效果。
| 生物活性 | γ-Aminobutyric acid (4-Aminobutyric acid) is a major inhibitory neurotransmitter in the adult mammalian brain, binding to the ionotropic GABA receptors (GABAAreceptors) and metabotropic receptors (GABABreceptors. γ-Aminobutyric acid shows calming effect by blocking specific signals of central nervous system[1][2]. |
| IC50& Target | Human Endogenous Metabolite | Microbial Metabolite |
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体外研究
(In Vitro) | γ-Aminobutyric acid (30 μM) depolarizes cortical progenitor cells (E16 cells), results an inward current in ventricular zone (VZ) cells, and induces DNA synthesis inhibition, with half-maximal response concentration of 5 μM[3].
Cortical plate (cp) neurons expresses glutamic acid decarboxylase (GAD), γ-Aminobutyric acid (1-5 μM; 18 h) stimulates the motility and arrests the migration of cp cells, while the chemotropic signal is involved G-protein activation[4].
γ-Aminobutyric acid activates GABAAreceptors, causing cell cycle arrest in S phase and limiting growth[5].
Cell Migration Assay[4] | Cell Line: | Cortical plate (cp) neurons | | Concentration: | 1-5 μM | | Incubation Time: | 18 hours | | Result: | Promoted motility via G-protein activation and arrested attractantinduced migration via GABAA receptor-mediated depolarization. |
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体内研究
(In Vivo) | γ-Aminobutyric acid (33.95, 102.25, 306.75 mg/kg; p.o.; single dose) can enhance the sleep of mice[6].
γ-Aminobutyric acid (1, 2, 4 mg/kg/d; p.o.; 30 d) alleviates anxiety and restored food utilization rate in rats, with impairment induced by Di(2-ethylhexyl) phthalate (DEHP) exposure[7].
| Animal Model: | Pathogens free (SPF) Bagg’s albino (Balb/c) mice (18–20 g, 8 weeks old)[6] | | Dosage: | 33.95, 102.25, 306.75 mg/kg | | Administration: | Oral gavage; single dose; 20 mL/kg administration; measured in an hour | | Result: | Prolonged the sleep duration, increased sleep rate, and shorten the sleep latency more effectively. |
| Animal Model: | Sprague-Dawley rat indued byDEHP(HY-B1945) (500 mg/kg)[7] | | Dosage: | 1, 2, 4 mg/kg | | Administration: | Oral gavage; combined administration; for 30 consecutive days | | Result: | Reduced the levels of nitric oxide and nitric oxide synthase in rats treated with DEHP. |
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| 结构分类 | - Ketones, Aldehydes, Acids
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| 来源 | mammalian central nervous system |
| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | | Powder | -20°C | 3 years | | 4°C | 2 years | | In solvent | -80°C | 6 months | | -20°C | 1 month |
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| 溶解性数据 | In Vitro: H2O : 50 mg/mL(484.87 mM;Need ultrasonic) 配制储备液 | 1 mM | 9.6974 mL | 48.4872 mL | 96.9744 mL | | 5 mM | 1.9395 mL | 9.6974 mL | 19.3949 mL | | 10 mM | 0.9697 mL | 4.8487 mL | 9.6974 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： PBS Solubility: 100 mg/mL (969.74 mM); Clear solution; Need ultrasonic and warming and heat to 60℃
*以上所有助溶剂都可在本网站选购。 |
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