Deoxypodophyllotoxin (DPT)，鬼臼毒素的衍生物，是一种木酚素，从Anthriscus sylvestris分离，具有强效抗有丝分裂和抗病毒特性。Deoxypodophyllotoxin 作为靶向微管的药物，不仅抗有丝分裂剂而且作为有效的血管生成抑制剂，在肿瘤学中具有重大影响。Deoxypodophyllotoxin 诱导细胞自噬 (autophagy) 和细胞凋亡 (apoptosis)。
Deoxypodophyllotoxin 引起 DRG 神经元细胞内 Ca2+浓度增加。
| 生物活性 | Deoxypodophyllotoxin (DPT), a derivative of podophyllotoxin, is a lignan with potent antimitotic, anti-inflammatory and antiviral properties isolated fromAnthriscus sylvestris. Deoxypodophyllotoxin, targets themicrotubule, has a major impact in oncology not only as anti-mitotics but also as potent inhibitors of angiogenesis[1]. Deoxypodophyllotoxin induces cellautophagyandapoptosis[2]. Deoxypodophyllotoxin evokes increase of intracellular Ca2+concentrations in DRG neurons[3]. |
体外研究
(In Vitro) | Deoxypodophyllotoxin (25-75 nM; 6-48 hours) increases the percentage of early apoptotic cell population from 2.05 to 5.62 and 18.49% for 24 h and 48 h, respectively[1].
Deoxypodophyllotoxin (25-75 nM; 6-48 hours) treats SGC-7901 cells arrested in G2/M phase in time- and dose- dependent manners[1].
Deoxypodophyllotoxin (25-75 nM; 6-48 hours) results in a remarkably time- and dose-dependent decrease in Cdc2 and Cdc25C expression levels and increases cyclin B1 within 6h, decreases PARP, Bcl-2 and caspase-3 activity[1].
Apoptosis Analysis[1] | Cell Line: | SGC-7901 cells | | Concentration: | 25, 50, 75 nM | | Incubation Time: | 6, 12, 24, 48 hours | | Result: | Induced apoptosis in SGC-7901 Cells. |
Cell Cycle Analysis[1] | Cell Line: | SGC-7901 cells | | Concentration: | 25, 50, 75 nM | | Incubation Time: | 6, 12, 24, 48 hours | | Result: | Induced G2/M cell cycle arrest in SGC-7901 Cells |
Western Blot Analysis[1] | Cell Line: | SGC-7901 cells | | Concentration: | 25, 50, 75 nM | | Incubation Time: | 6, 12, 24, 48 hours | | Result: | Altered the expression of cyclin B1, Cdc2,Cdc25C, p-PARP, Bcl-2 and p-caspase-3 proteins. |
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体内研究
(In Vivo) | Deoxypodophyllotoxin (intravenously injected; 5, 10, and 20 mg/kg; 3 times a week; 28 days) suppresses the tumors in a dose-dependent manner, the growth of tumors is inhibited by 22.19%, 47.91% and 50.93% with DPT at 5, 10 and 20 mg/kg, respectively[1].
| Animal Model: | Xenograft model of gastric cancer in nude mice with SGC-7901 cells[1] | | Dosage: | 5, 10, and 20 mg/kg | | Administration: | Intravenously injected; 5, 10, and 20 mg/kg; 3 times a week; 28 days | | Result: | Inhibited the growth of gastric cancer tumors. |
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| 来源 | - Plants
- Umbelliferae
- Anthriscus sylvestris
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | | Powder | -20°C | 3 years | | 4°C | 2 years | | In solvent | -80°C | 6 months | | -20°C | 1 month |
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| 溶解性数据 | In Vitro: DMSO : 100 mg/mL(251.00 mM;Need ultrasonic) 配制储备液 | 1 mM | 2.5100 mL | 12.5499 mL | 25.0998 mL | | 5 mM | 0.5020 mL | 2.5100 mL | 5.0200 mL | | 10 mM | 0.2510 mL | 1.2550 mL | 2.5100 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： 10% DMSO 40%PEG300 5%Tween-80 45% saline Solubility: ≥ 2.5 mg/mL (6.27 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (6.27 mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。 2. 请依序添加每种溶剂： 10% DMSO 90% (20%SBE-β-CDin saline) Solubility: ≥ 2.5 mg/mL (6.27 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (6.27 mM，饱和度未知) 的澄清溶液。 以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。 3. 请依序添加每种溶剂： 10% DMSO 90%corn oil Solubility: ≥ 2.5 mg/mL (6.27 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (6.27 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。 以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。
*以上所有助溶剂都可在本网站选购。 |
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