质粒名称:pThermoCas9_ctrl
质粒编号:100981
质粒备注:Expresses ThermoCas9 and its sgRNA module
载体名称:pNW33n
载体大小:4217
完整质粒大小:7467
载体类型:原核表达
原核抗性:氯霉素25μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:未知
插入基因:Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
基因别名:ThermoCas9
物种来源:Geobacillus thermodenitrificans T12
插入大小:3249
启动子:B. smithii xylL promoter
克隆方法:Gibson Cloning
5'测序引物:ATGAAGTATAAAATCGGTCTTG
3'测序引物:TTATAACGGACGGATAGTTTC
质粒图谱: