质粒名称:M51 Super 8x FOPFlash (TOPFlash mutant)
质粒编号:12457
质粒备注:Control for beta-catenin reporter. Contains mutated TCF/LEF binding sites upstream of a luciferase reporter.
载体名称:pGL3
载体大小:4839
载体类型:Mammalian Expression, Luciferase
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:mutant TCF/LEF binding sites
基因别名:beta catenin reporter
基因突变:mutant TCF/LEF binding sites
插入大小:134
基因名称:Ctnnb1 (a.k.a. Bfc, Catnb, Mesc)
克隆方法:限制性内切酶
5'克隆位点:Asp718 (not destroyed)
3'克隆位点:XmaI (not destroyed)
5'测序引物:RVPrimer3
3'测序引物:LucNRev
质粒图谱: