质粒名称:pT040
质粒编号:67640
质粒备注:URA3 vector for cloning guide sequences using BclI-SwaI sites into guide RNA expression cassette
载体名称:pCT3
载体大小:4749
完整质粒大小:5187
载体类型:Yeast Expression, CRISPR
筛选标记:URA3
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:single guide RNA expression cassette
物种来源:S. cerevisiae (budding yeast), Synthetic
启动子:pSNR52
克隆方法:限制性内切酶
5'克隆位点:SacII (not destroyed)
3'克隆位点:EagI (not destroyed)
5'测序引物:T3
质粒图谱: