质粒名称:pRS300
质粒编号:22846
质粒备注:Backbone for expressing plant artificial miRNAs
载体名称:pBluescriptSK+
载体大小:2964
载体类型:Plant Expression, RNAi ; Plant gene inactivation
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:MIR319a
物种来源:A. thaliana (mustard weed)
插入大小:404
基因名称:MIR319a (a.k.a. AT4G23713, JAGGED AND WAVY, JAW, MICRORNA 319, MIR319, MIR319A, microRNA319A, p_MI0000544)
克隆方法:限制性内切酶
5'克隆位点:Sma1 (destroyed during cloning)
3'克隆位点:Sma1 (destroyed during cloning)
5'测序引物:T7
3'测序引物:T3
质粒图谱: