质粒名称:pRCC-K
质粒编号:81191
质粒备注:Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance
载体名称:pRS42K
载体类型:Yeast Expression, CRISPR
筛选标记:新霉素(G418)
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:Cas9
物种来源:S. pyogenes
基因突变:WT - codon optimized
启动子:ROX3
克隆方法:Gibson Cloning
5'测序引物:unknown
质粒图谱: