质粒名称:pNHEJ-RPG
质粒编号:85931
质粒备注:Constitutively express DsRed and conditionally express Puro and EGFP. Used to test transfection efficiency and enrich genetically modified cells
载体名称:pXL-Bac II
载体类型:Mammalian Expression ; dual-reporter surrogate
筛选标记:嘌呤霉素
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:pPB-CMV-DsRed-polyA-CAG-NHEJ. Puro-T2A-EGFP
基因别名:DsRed, CAG-NHEJ. Puro-T2A-EGFP
克隆方法:未知
5'测序引物:pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3')
3'测序引物:SV40pA-R
质粒图谱: