质粒名称:pMSCVpuro-Mito-Pericam
质粒编号:87381
质粒备注:Retroviral transfection of cells to express ratiometric Pericam as described by Nagai et al 2001 (PMID 11248055) localising to the mitochondrial matrix via the transit peptide from human COX8A.
载体名称:pMSCVpuro
载体大小:6264
完整质粒大小:7740
载体类型:哺乳动物表达,逆转录
筛选标记:嘌呤霉素
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:NEB Stable
高拷贝/低拷贝:高拷贝
插入基因:Mito-Pericam [ratiometric]
插入大小:1488
启动子:Retroviral 5' LTR promoter
克隆方法:限制性内切酶
5'克隆位点:NheI (not destroyed)
3'克隆位点:EcoRI (not destroyed)
5'测序引物:pMSCV-F
3'测序引物:pMSCV-R
质粒图谱: