质粒名称:pEVOL-pBpF
质粒编号:31190
质粒备注:tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-benzoyl-l-phenylalanine into proteins in E coli in response to the amber codon, TAG.
载体名称:p15A
载体大小:5000
载体类型:原核表达
原核抗性:氯霉素25μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:M.j. p-benzoylphenylalanine RS (2 copies +tRNA)
基因别名:pEVOL-pBpF
物种来源:M.jannaschii
基因突变:Y32G, E107P, D158T, I159S
插入大小:1000
克隆方法:限制性内切酶
5'克隆位点:BglII (not destroyed)
3'克隆位点:SalI (not destroyed)
5'测序引物:ATTAGCGGATCCTACCTGACGCTTTTTATCGCAACTCTCTACTGTTTCTCCATACCCGTTTTTT
质粒图谱: