质粒名称:pdCas9-Tet1-CD
质粒编号:83340
质粒备注:To introduce dCas9-fused Tet1-CD and sgRNA2.0 system
载体名称:pX330
载体类型:哺乳动物表达,CRISPR
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:NEB Stable
高拷贝/低拷贝:高拷贝
插入基因:dCas9-Tet1-CD and sgRNA scaffold with 2xMS2 binding sites
物种来源:Synthetic
基因突变:D10A, H840A
启动子:U6, CBH
克隆方法:Gibson Cloning
5'测序引物:U6
3'测序引物:BGH-rev
质粒图谱: