质粒名称:pCRCT
质粒编号:60621
质粒备注:Plasmid encoding iCas9, tracrRNA and crRNAs
载体名称:pRS426
载体大小:5000
完整质粒大小:10805
载体类型:Yeast Expression, CRISPR
筛选标记:URA3
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:iCas9
物种来源:Streptococcus pyogenes
基因突变:changed Aspartate 147 to Tyrosine, Proline 411 to Threonine
插入大小:4179
启动子:TEF1p
标签/融合蛋白:· FLAG (N terminal on insert)
· SV40 NLS (N terminal on insert)
· SV40 NLS (C terminal on insert)
克隆方法:Ligation Independent Cloning
5'测序引物:Unknown
3'测序引物:Unknown
质粒图谱: