质粒名称:pALOD4
质粒编号:111026
质粒备注:Expresses domain 4 of anthrolysin O cholesterol sensor, has N-terminal His6-tag and two point mutations, S404C and C472A
载体名称:pRSET-B
载体大小:2887
载体类型:原核表达
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:未知
插入基因:Domain 4 of Anthrolysin O
基因别名:ALOD4
物种来源:Bacillus Anthracis
基因突变:Mutations: S404C, C472A
插入大小:429
启动子:T7
克隆方法:限制性内切酶
5'克隆位点:BamH1 (unknown if destroyed)
3'克隆位点:EcoR1 (unknown if destroyed)
5'测序引物:TAA TAC GAC TCA CTA TAG GG
3'测序引物:GCT AGT TAT TGC TCA GCG G
质粒图谱: