质粒名称:lenti-EF1a-dCas9-KRAB-Puro
质粒编号:99372
质粒备注:3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)
载体名称:pLenti
载体大小:9192
完整质粒大小:14337
载体类型:哺乳动物表达,慢病毒,CRISPR
筛选标记:Puromycin, Zeocin
原核抗性:氨苄青霉素,100μg/mL
培养温度:30℃
克隆菌株:NEB Stable
高拷贝/低拷贝:高拷贝
插入基因:dCas9-KRAB-T2A-Puro
物种来源:synthetic; S. pyogenes
插入大小:5145
启动子:EF-1a
克隆方法:Gibson Cloning
5'测序引物:GTTTGGATCTTGGTTCATTCTCAAGCCTCAG
3'测序引物:cacatagcgtaaaaggagcaacatag
质粒图谱: