质粒名称:lenti sgRNA(MS2)_puro backbone
质粒编号:73795
质粒备注:(Empty Backbone) 3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
载体名称:plenti
载体大小:10221
载体类型:哺乳动物表达,慢病毒,CRISPR
筛选标记:嘌呤霉素
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:Stbl3
高拷贝/低拷贝:高拷贝
启动子:U6 and EF1A
克隆方法:限制性内切酶
5'测序引物:GAG GGC CTA TTT CCC ATG ATT CCT TCA TAT
3'测序引物:cctagaaggtccattagctgcaaagattcc
质粒图谱: