质粒名称:lenti MS2-P65-HSF1_Hygro
质粒编号:61426
质粒备注:lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2
载体名称:plenti
载体类型:哺乳动物表达,慢病毒,CRISPR
筛选标记:潮霉素
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:Stbl3
高拷贝/低拷贝:高拷贝
插入基因:MS2-P65-HSF1_2A_Hygro
物种来源:H. sapiens (human), Synthetic
基因突变:N55K in MS2
基因名称:HSF1 (a.k.a. HSTF1)
启动子:EF1A
克隆方法:限制性内切酶
5'克隆位点:BsiWI (not destroyed)
3'克隆位点:EcoRI (not destroyed)
5'测序引物:GTT TGG ATC TTG GTT CAT TCT CAA GCC TCA G
3'测序引物:cacatagcgtaaaaggagcaacatag
质粒图谱: