质粒名称:Laconic/pcDNA3.1(-)
质粒编号:44238
质粒备注:a genetically-encoded sensor for lactate concentration, transport and metabolic flux
载体名称:pcDNA3.1(-)
载体大小:5418
完整质粒大小:7648
载体类型:Mammalian Expression ; lactate biosensor
筛选标记:新霉素(G418)
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:Laconic (LACtate Optical Nano Indicator from CECs)
物种来源:Synthetic
插入大小:2230
启动子:CMV
克隆方法:限制性内切酶
5'克隆位点:BamHI (unknown if destroyed)
3'克隆位点:HindIII (unknown if destroyed)
5'测序引物:CMV-F
3'测序引物:BGH-rev
质粒图谱: