质粒名称:GST-BHMT-I/G
质粒编号:104442
质粒备注:Autophagy cargo assay
载体名称:pRK5
载体大小:4754
完整质粒大小:7832
载体类型:哺乳动物表达
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:DH5alpha
高拷贝/低拷贝:高拷贝
插入基因:BHMT
物种来源:H. sapiens (human)
基因突变:N43A in GST (in a potential KFERQ-like sequence). BHMT does not contain the first 8 or last 9 amino acids.
插入大小:3213
基因名称:BHMT (a.k.a. BHMT1, HEL-S-61p)
启动子:CMV
标签/融合蛋白:· GST (N terminal on insert)
· GFP-myc GFP is from Aequorea victoria (C terminal on insert)
克隆方法:限制性内切酶
5'克隆位点:Nru1 (unknown if destroyed)
3'克隆位点:HindIII (unknown if destroyed)
5'测序引物:SP6 5'-TGATGAGTTTGGACAAACCAC-3'
质粒图谱: