质粒名称:Fuw-dCas9-Dnmt3a_IM
质粒编号:84478
质粒备注:Lentiviral construct to express dCas9 fused with an inactive Dnmt3a catalytic domain (E664A, E756A)
载体名称:FUW
载体大小:9207
完整质粒大小:14313
载体类型:哺乳动物表达,慢病毒
筛选标记:Zeocin ; Resistance marker is outside the LTRs and will not be packaged into virus
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:NEB Stable
高拷贝/低拷贝:高拷贝
插入基因:dCas9-Dnmt3a_IM
物种来源:H. sapiens (human), Synthetic
插入大小:5106
克隆方法:限制性内切酶
5'克隆位点:AscI (not destroyed)
3'克隆位点:EcoRI (not destroyed)
5'测序引物:aactatgcgctcggg
3'测序引物:taaagcagcgtatcc
质粒图谱: