质粒名称:BPK764
质粒编号:65767
质粒备注:Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA
载体名称:pACYCDuet-1
载体类型:Bacterial Expression, CRISPR
原核抗性:氯霉素25μg/mL
培养温度:37℃
克隆菌株:XL1 Blue
高拷贝/低拷贝:低拷贝
插入基因:mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
启动子:T7 (x2)
标签/融合蛋白:· NLS (C terminal on insert)
· 3x FLAG (C terminal on insert)
克隆方法:Gibson Cloning
5'测序引物:ACYC-DuetUP1
3'测序引物:oBK390 (5'-GCAAATTCGACCCGGTCGTCG-3')
质粒图谱: