质粒名称:6His-MBP-TEV-huAsCpf1
质粒编号:90095
质粒备注:Bacterial expression plasmid for protein purification
载体名称:pET-28
载体大小:6643
完整质粒大小:10710
载体类型:原核表达
原核抗性:氨苄青霉素,100μg/mL
培养温度:37℃
克隆菌株:BL21(DE3)pLysS
高拷贝/低拷贝:低拷贝
插入基因:huAsCpf1
物种来源:humanized Acidaminococcus sp. BV3L6 Cpf1
插入大小:4056
标签/融合蛋白:· 6xHis (N terminal on backbone)
· MBP (N terminal on insert)
· TEV site (N terminal on insert)
· Nucleoplasmin NLS (C terminal on insert)
· 3xHA (C terminal on insert)
质粒图谱: